Biomedical Lab Sci

Clinical Laboratorial Biochemistry I2

Bioquímica Clínica laboratorial I

Course code:68301

Maria João Pimenta Hilário

Learning outcomes of the curricular unit:

The Curricular Unit of Clinical Laboratorial Biochemistry I is based on biochemical knowledge that allows students to interpret and interconnect sub-cellular, cellular and physiological processes as well as the metabolic sequences involved in homeostasis and functions of organs and systems. After attending the CU the student should be able to:

  • Have knowledge about the role of the clinical biochemistry in the diagnostics and in the treatment of disease, as well as the importance of laboratory in diagnosis and monitoring of specific disease conditions.
  • Use the concepts and techniques taught
  • Have the ability to work neatly and in a team
  • Carry out scientific research in this area
  • Know and execute the methodologies for determining various analytes expressed in analytical values
  • Interpret the analyse changes envisaged in pathology
  • Be prepared to do the evaluation and integration of clinical and laboratorial findings
  • Execute the laboratory control of analytical determination
Syllabus:
  1. RELATIONSHIP - PATHOLOGY AND BIOCHEMICAL CHANGES
  2. PROTEINS
  3. NON-PROTEIN NITROGEN COMPOUNDS
  4. CARBOHYDRATES
  5. LIPIDS
  6. BLOOD GASES AND ACID-BASE BALANCE
  7. ORGANIC IONS
LAB PRACTICE CLASSES
Spectrophotometry, photometry, turbidimetry and nephelometry
Determination of serum levels of total proteins and albumin: Alternative methods; Clinical interest
Determination of serum levels of urea and uric acid: Alternative methods; Clinical interest
Determination of serum creatinine levels: Alternative methods; Clinical interest
Determination of serum glucose levels: Alternative methods; Clinical interest. Postprandial glucose and glycemic curve: practical procedure.
Determination of serum levels of Cholesterol and Triglycerides: Alternative methods; Clinical interest.
Determination of serum levels of serum ionogram: Alternative methods; Clinical interest.
Relationship between pathology and biochemical changes.
Quality control.
 
Suggested Bibliography:
  • Bishop ML et al (2013) Clinical Chemistry: Principles, Techniques, and Correlations, 7th ed., Lippincott Williams and Wilkins.
  • Burtis CA and Bruns DE (2014). Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 7th ed., Saunders
  • Burtis CA et al. (2007) Tietz Fundamentals of Clinical Chemistry, 6th ed., Saunders
  • Kaplan LA & Pesce AJ (2009). Clinical Chemistry – Theory, Analysis, Correlation, 5th ed., Mosby