Study Programmes

Animal Organism II – From Cell to Tissueveterinary MIMV

Organismo Animal II – Da Célula ao Tecido

Course code: 9847103

Alexandre José Neto Trindade

Learning outcomes of the curricular unit:
  • Analyze the cell as a unit integrated in a biological system that responds to external stimuli of cellular signaling.
  • Understand the mechanisms inherent to cell cycle regulation and implications on the functioning and homeostasis of the living system.
  • Acquire knowledge and an integrated view of hormonal regulation mechanisms and metabolic interrelations.
  • Consolidation of knowledge about the importance of scientific methodology in the study of veterinary medicine.
Syllabus:
    1. Cell signaling pathways and their importance in the regulation of tissues and organs: functional significance. Signaling forms: Paracrine, Autocrine, Synaptic, Endocrine - examples and functional importance.
    2. Vesicular transport. Endocytosis. Exocytosis. Lysosomes: Structure and Function.
    3. The cell cycle. Cell Cycle Regulation
    4. Programmed cell death. Necrosis versus Apoptosis. Concepts of regulated necrosis, necroptosis and autophagy. Biochemical mechanisms in cell death and proliferation.
    5. Metabolism Regulation and Integration :: main metabolic pathways and hormonal regulation.
    6. Hormonal regulation: Aspects related to hormonal actions in different tissues.
    7. Lifelong metabolic disorders and pathological changes in metabolism.
    8. Microscopy and immunohistochemical techniques for visualization of animal cells and tissues.
    9. Chromatographic techniques. Protein analysis techniques.
    10. Clinical biochemistry - enzyme immunoassay (ELISA).
Practice Component
  1. Cell culture basics
  2. Analysing the cell cycle
  3. Chromatographic techniques (paper chromatography)
  4. Using immunocytochemistry to explore anti-cancer drugs effect on the cell cycle
  5. Chromatographic techniques (ion exchange chromatography)
  6. Learning about protein colocalization
  7. Clinical biochemistry - enzyme immunoassay (ELISA).
  8. A case study approach to signalling pathways
  9. Protein analysis techniques
  10. Investigating signalling pathways with gene expression datasets
  11. Protein analysis techniques
Suggested Bibliography:
  • Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K. & Walter, P. (2014) - Molecular Biology of the Cell. 6th edition. New York : Garland Science
  • David L. Nelson, Michael M. Cox (2021). Lehninger Principles of Biochemistry. Eighth Edition. W.H.FREEMAN & CO LTD

 

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