Animal Organism II – From Cell to Tissue
Organismo Animal II – Da Célula ao Tecido
Course code: 9847103
Alexandre José Neto Trindade
Learning outcomes of the curricular unit:
- Analyze the cell as a unit integrated in a biological system that responds to external stimuli of cellular signaling.
- Understand the mechanisms inherent to cell cycle regulation and implications on the functioning and homeostasis of the living system.
- Acquire knowledge and an integrated view of hormonal regulation mechanisms and metabolic interrelations.
- Consolidation of knowledge about the importance of scientific methodology in the study of veterinary medicine.
Syllabus:
- Cell signaling pathways and their importance in the regulation of tissues and organs: functional significance. Signaling forms: Paracrine, Autocrine, Synaptic, Endocrine - examples and functional importance.
- Vesicular transport. Endocytosis. Exocytosis. Lysosomes: Structure and Function.
- The cell cycle. Cell Cycle Regulation
- Programmed cell death. Necrosis versus Apoptosis. Concepts of regulated necrosis, necroptosis and autophagy. Biochemical mechanisms in cell death and proliferation.
- Metabolism Regulation and Integration :: main metabolic pathways and hormonal regulation.
- Hormonal regulation: Aspects related to hormonal actions in different tissues.
- Lifelong metabolic disorders and pathological changes in metabolism.
- Microscopy and immunohistochemical techniques for visualization of animal cells and tissues.
- Chromatographic techniques. Protein analysis techniques.
- Clinical biochemistry - enzyme immunoassay (ELISA).
Practice Component
- Cell culture basics
- Analysing the cell cycle
- Chromatographic techniques (paper chromatography)
- Using immunocytochemistry to explore anti-cancer drugs effect on the cell cycle
- Chromatographic techniques (ion exchange chromatography)
- Learning about protein colocalization
- Clinical biochemistry - enzyme immunoassay (ELISA).
- A case study approach to signalling pathways
- Protein analysis techniques
- Investigating signalling pathways with gene expression datasets
- Protein analysis techniques
Suggested Bibliography:
- Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K. & Walter, P. (2014) - Molecular Biology of the Cell. 6th edition. New York : Garland Science
- David L. Nelson, Michael M. Cox (2021). Lehninger Principles of Biochemistry. Eighth Edition. W.H.FREEMAN & CO LTD
Additional Info
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