Biomedical Lab Sci

Clinical and Laboratorial Virology1

Virologia Clinico-Laboratorial

Course code:68309

Teresa Maria Silva do Nascimento

Learning outcomes of the curricular unit:

The CU aims to provide students with knowledge in general viruses characteristics, morphology, composition, classification, replication, interaction between virus and cell, pathogenesis, prevention, treatment and Virology quality control. At the end of the CU students should be able to:

  • Demonstrate to know the fundamental concepts of Virology;
  • Know that the emergence of new viral strains place huge demands on healthcare systems;
  • Demonstrate knowledge of the methodologies used in laboratory diagnosis and monitoring of the main virus associated with diseases in humans;
  • Analyse experimental protocols;
  • Perform the techniques trained in classes with autonomy;
  • Contextualize the results obtained;
  • Argue their findings using the appropriate terminology;
  • Carry out scientific research;
  • Make reports and oral presentation/discussion;
  • Have critical thinking;
  • Know how to avoid technical and diagnostic errors;
  • Demonstrate to know the standard of control quality in Virology laboratory.

Students who successfully complete the course must have acquired

  1. adequate knowledge of the subject taught
  2. the ability of interpretation in relation to situations related to the subjects taught including interpretation and evaluation of clinical virological requirements and subsequent execution
  1. Concepts of Virology. Epidemiology. Virus structure, composition, classification and nomenclature.Mechanisms of replication and viral pathogenesis.
  2. Clinical Virology: pathogenesis, clinical, epidemiological and laboratory diagnosis.
  3. Human herpesvirus. HSV- 1/2 and VZV, CMV, EBV and viral hepatitis.
  4. Poxvirus capable of causing disease in humans. The smallpox virus and its eradication. Molluscum contagiosum vírus.
  5. Clinical and diagnostic aspects of virus associated with acute diarrheal disease and with respiratory disease.
  6. Human retroviruses: HTLV-I/II and the human immunodeficiency virus, HIV-1/HIV-2.
  7. Oncogenic Viruses and prions.
Laboratory safety.
Quality control.
Pre-analytical variables: harvesting, storage and transport of biological products.
Direct and indirect laboratory methods of viral infections.
Diagnosis of infection with Herpes simplex, cytomegalovirus, rubella, hepatitis B, HPV, HIV -1 and HIV-2.
Suggested Bibliography:
  • Barroso H, Meliço-Silvestre A, Taveira N (2014) Microbiologia Médica Volume 1 e 2, Lidel ISBN:9789727575763;
  • Collier L, Oxford J, Kellam P (Eds) (2011) Human Virology, 4th Ed. Oxford University Press. ISBN: 0127466428
  • Storch GA (2000) Essentials of Diagnostic Virology. Churchill Livingstone. ISBN 978-0443076329