Biomedical Lab Sci

Clinical and Laboratorial Microbiology II2

Microbiologia Clinico-Laboratorial II

Course code:68212

Teresa Maria da Silva do Nascimento

Learning outcomes of the curricular unit:

At the end of this course the student should know the general course of microbiological analysis of different organic products, in order to identify and test sensitivity to drugs (antibiotics and antifungals) for different microorganisms potentially pathogenic to humans.

Students who successfully complete the course must have acquired:

  • Adequate knowledge of the subject taught.
  • The ability of reasoning and interpretation in relation to situations related to the subjects taught including interpretation and evaluation of clinical microbiological requirements and respective execution.
    1. Cytology, bacteriology aseptic urine: laboratory procedures: collection, transportation, direct examination, culture and sensitivity test. Detailed analysis of aseptic urine. Scheme procedures
    2. Interpretation of laboratory results in aseptic urine: dysuria, pyuria and bacteriuria. Validation criteria. Search fastidious microorganisms (BK).
    3. Microbiological examination of faecal matter: Dinner flora of the gastrointestinal tract, types of infection: gastroenteritis, enterocolitis, diarrhea. Gastrointestinal infection. Pathophysiological mechanisms.
    4. Pathogenic and potentially pathogenic microorganisms in the female and male genital tract. Factors that predispose to genital infection, modes of transmission Notion of Sexually Transmitted Infection (IST) Epidemiology and prevention.
    5. Etiological agents potentially pathogenic vaginal and urethral exudate. Cytology, bacteriology of vaginal and urethral exudates: laboratory procedures: collection, transportation, direct examination, culture, antibiotics sensitivity test and brief reference to treatment. Classic microbiological diagnostics and molecular methods for the detection of Chlamydia, Mycoplasma, and Treponema.
    6. Microbiological examination of upper and lower respiratory tract samples.
    7. Types of bacteremia, blood culture: the reference manual and automated processes, clinical interpretation of the results of blood cultures.
    8. Structure according to the meninges. Functions of liquor. Routes of infection of the nervous system. Microorganisms (bacteria, viruses and fungi) responsible for meningitis. Classification of meningitis. Laboratory diagnosis of CSF.
Suggested Bibliography:
      • Barroso H, Silvestre AM & Taveira N (2014) Microbiologia Médica Volume 1 e 2, Lidel. ISBN:9789727575763
      • Mims C, Playfair J, Roitt I, Wakelin D & Williams R (2013) Medical Microbiology, 5th ed., Mosby. ISBN: 9780808924401
      • Murray PR, Kobayashi GS, Pfaller MA & Rosenthal KS (2016) Medical Microbiology, 8th ed., Mosby. ISBN: 9780323359498
      • Tille P (2018) Diagnostic Microbiology, 14th ed., Bailey´s & Scott´s. ISBN: 9780323354820