Histotechnology II
Histotecnologia II
Course code:68214
Pedro José Nina Lopes
Learning outcomes of the curricular unit:
- Recognize and interpret the role of an histotechnologist in the laboratory
- Recognize and apply in a correct way all terminology used in Histotechnology
- Recognize and be able to handle all necessary equipment for carrying out tasks in this professional area
- Follow the work methodology applied in the laboratory
- Plan, apply and interpret specific techniques of Histotechnology, in order to enable microscopic observation
- Develop studies and participate in Histotechnology investigation projects
- Demonstrate skills to select and develop techniques which support diagnostic
- Develop macroscopic and/or microscopic examination of cells/tissues as disease indicators
- Demonstrate the ability to collect and select the appropriate biological samples and the relevant procedures for clinical and laboratory needs
Syllabus:
- Staining theory
- Staining dyes
- Factors that influence staining distribution in tissues
- General staining mechanisms
- Nuclear staining dyes / hematoxilins
- Citoplasmatic staining dyes / eosin
- Mounting media
- Errors in histological techniques
- Archives
- Toxic risks and responsibilities of the Pathological Anatomy analists
- Quality Control
- Electronic Microscopy
- Plastination and other techniques of conservation of organs for museology and teaching
Suggested Bibliography:
- Carson FL (2015) Histotechnology - A Self-Assessment Workbook, 3rd ed., ASCP Press.
- Carson FL and Cappellano CH (2015) Histotechnology – a Self-Instructional Text, 4th ed., American Society of Clinical Pathologists Press.
- Orchard G and Nation B (2012) Histopathology (Fundamentals of Biomedical Science). OUP Oxford.
- Moral RG (1993) Laboratorio de Anatomía Patológica, McGraw-Hill, interamericana.
- Suvarna SK, Layton C and Bancroft JD (2013) Bancroft's Theory and Practice of Histological Techniques, 7th ed., Churchill Livingstone, Elsevier.