Biomedical Lab Sci

Histotechnology II1

Histotecnologia II

Course code:68214

Pedro José Nina Lopes

Learning outcomes of the curricular unit:
  • Recognize and interpret the role of an histotechnologist in the laboratory
  • Recognize and apply in a correct way all terminology used in Histotechnology
  • Recognize and be able to handle all necessary equipment for carrying out tasks in this professional area
  • Follow the work methodology applied in the laboratory
  • Plan, apply and interpret specific techniques of Histotechnology, in order to enable microscopic observation
  • Develop studies and participate in Histotechnology investigation projects
  • Demonstrate skills to select and develop techniques which support diagnostic
  • Develop macroscopic and/or microscopic examination of cells/tissues as disease indicators
  • Demonstrate the ability to collect and select the appropriate biological samples and the relevant procedures for clinical and laboratory needs
  1. Staining theory
  2. Staining dyes
  3. Factors that influence staining distribution in tissues
  4. General staining mechanisms
  5. Nuclear staining dyes / hematoxilins
  6. Citoplasmatic staining dyes / eosin
  7. Mounting media
  8. Errors in histological techniques
  9. Archives
  10. Toxic risks and responsibilities of the Pathological Anatomy analists
  11. Quality Control
  12. Electronic Microscopy
  13. Plastination and other techniques of conservation of organs for museology and teaching


Suggested Bibliography:
  • Carson FL (2015) Histotechnology - A Self-Assessment Workbook, 3rd ed., ASCP Press.
  • Carson FL and Cappellano CH (2015) Histotechnology – a Self-Instructional Text, 4th ed., American Society of Clinical Pathologists Press.
  • Orchard G and Nation B (2012) Histopathology (Fundamentals of Biomedical Science). OUP Oxford.
  • Moral RG (1993) Laboratorio de Anatomía Patológica, McGraw-Hill, interamericana.
  • Suvarna SK, Layton C and Bancroft JD (2013) Bancroft's Theory and Practice of Histological Techniques, 7th ed., Churchill Livingstone, Elsevier.