Biomedical Lab Sci

Clinical and Laboratorial Haematology II2

Hematologia Clínico-Laboratorial II

Course code:68211

Teresa Maria Silva do Nascimento

Learning outcomes of the curricular unit:

At the end of this course, the student should know: The physiology of white blood cell, the leucopoiesis, their benign and malignant changes and also the mechanisms of hemostasis and laboratory parameters for their evaluation. At the end of the practical component the student will work in a hematology laboratory and perform basic hematological techniques, as well as current techniques coagulation, such as determination of prothrombin time, activated partial thromboplastin time, among others.

  1. Considerations about the different leukocyte populations: their physiology and functions performed. Qualitative changes of benign leukocytes: nucleus and cytoplasm. Benign quantitative changes of leukocytes. Pathogenesis and hematologic laboratory diagnosis. Interpretation of some clinical cases.
  2. Acute leukemia: WHO (FAB classification) Epidemiology, clinical, laboratory diagnostics, immunophenotyping and cytogenetics. Chronic myeloproliferative diseases. Chronic lymphoproliferative disorders. Malignancies T and B cells Evidence of monoclonal B cells by immunophenotyping. Chronic lymphocytic leukemia, hairy cell leukemia. Lymphomas and multiple myeloma. Myelodysplastic syndromes. Laboratory diagnosis, brief reference to clinical and treatment. Hemostasis - general principles. Definition of hemostasis; intervening factors; Primary Hemostasis; Physiology of coagulation. Fibrinolytic system. Exploration of laboratory coagulation.
Analysis of histograms with pathological alterations in the red series. Importance of reference ranges. Performing venous blood collection. Execution and staining of peripheral blood smears: Leishman staining and MGG staining.
Thrombocytes. Platelet count and pseudotrombocytopenias. Realization and analysis of total blood cell counts.
Manual and automated hemostasis: determination of prothrombin time, activated partial thromboplastin time, and fibrinogen.
Cytochemical stains: Perls and Sudan Black.
Suggested Bibliography:
  • Dacie JV, Lewis SM (2011) Practical Haematology; 11th edition, Churchill Livingstone. ISBN: 978-0-7020-4862-3
  • Hoffbrand AV, Petit J (2016) Essencial Haematology, 7th edition, Wiley-Blackwell Science. ISBN: 978-1-118-40863-6
  • Hoffbrand AV, Petit J (2013) Colour Atlas of Clinical Haematology, 4th edition, Mosby. ISBN:978-0-3230-4453-0
  • Hoffman R. et al (2013) Haematology Basic Principles and Practice; 6rd edition, Churchill Livingstone. ISBN: 978-1-4377-2928-3
  • Kottke-Marchant K, Davis B (2012) Laboratory Hematology Practice; 1st edition, Wiley-Blackwell. ISBN: 978-1-4051-6218-0