Molecular Pathology

Patologia Molecular

Course code:68306

Maria Alexandra Taquenho Maia e Silva

Learning outcomes of the curricular unit:

The CU aims to consolidate the knowledge obtained in Molecular Biology and applied it to the diseases at the molecular level. After attending the course, students should be able to:

• Demonstrate knowledge about molecular diagnostic techniques
• Execute molecular diagnostic techniques trained at classes and Know the necessary equipment
• Select and evaluate molecular diagnostic techniques
• Use different substrates and biological samples
• Have critical thinking
• Demonstrate ability to analyse scientific papers that focus on diagnosis of molecular pathologies
• Discuss experimental results using the proper terminology
• Participate in a teamwork / projects related to molecular diagnostic techniques

Syllabus:

1. Analysis of nucleic acids and proteins
2. Genome analysis
3. Analysis of interactions between DNA and proteins
4. Molecular markers and genetic diseases
5. Analysis of mutations
6. Analysis of DNA markers
7. Genomic DNA
8. Molecular hybridizations and in situ hybridizations
9. Fish vs CISH
10. Epigenetics
11. Molecular pathologies associated with deficiencies in DNA repair mechanisms
12. Oncogenes and cancer suppressor genes

Suggested Bibliography:

• Coleman WB & Tsongalis GJ (2009) Molecular Pathology: The Molecular Basis of Human Disease, Academic Press, Elsevier.
• Coleman WB & Tsongalis GJ (2010) Essential Concepts in Molecular Pathology, Academic Press, Elsevier.
• Liang Cheng DZ (2008) Molecular Genetic Pathology, New Jersey, Human Press.