Biomedical Lab Sci

Clinical and Laboratorial Microbiology I2

Microbiologia Clinico-Laboratorial I

Course code:68202

Teresa Maria da Silva do Nascimento

Learning outcomes of the curricular unit:

Knowledge about pathogenesis, pathology, epidemiology, prevention and treatment of major human microbial infections. Integrated study of bacteria, viruses and fungi in different biological products, with particular emphasis is given to laboratory diagnosis of these infections.
At the end of this course the student should know the general course of microbiological analysis of different biological products, in order to identify and patch test sensitivity to drugs (antibiotics and antifungals) for different microorganisms potentially pathogenic to humans.

Students who successfully complete the course must have acquired:

  • Adequate knowledge of the subject taught.
  • The ability of interpretation in relation to situations related to the subjects taught including interpretation and evaluation of clinical microbiological requirements and subsequent execution.
Syllabus:
  1. DISTRIBUTION OF THE NORMAL FLORA OF THE HOST AND PARASITE-HOST RELATIONSHIP
  2. ANTIBIOTIC THERAPY
    Mechanisms of action.
    Resistance.
    Major antibiotics.
  3. FUNGI
    General characteristics of fungi.
    Classification of fungi.
    Fungus-host relationship.
    Antifungals.
    Major fungi responsible for fungal infections.
    Classification of mycoses.
    Etiology, pathogenesis, clinical presentation, laboratory diagnosis and therapy of primary cutaneous and mucocutaneous mycoses, subcutaneous and systemic mycoses.
  4. BACTERIOLOGY
    Bacterial infection.
    Main bacterial agents of clinical significance in humans.
  5. CLINICAL MICROBIOLOGY
    Analysis steps.
    Applied Clinical Microbiology: Microbiological analysis of upper and lower respiratory tract.
Lab practical classes:
 
Direct examination and culture of potentially pathogenic microorganisms.
Execution of antibiotic susceptibility test by different methodologies.
Protocols for different collections biological products.
General course of diagnosis of mycobacteria.
Mycological examination and culture of fungi.
Applied Clinical Microbiology: Microbiological analysis of upper and lower respiratory tract.
 
Suggested Bibliography:
  • Barroso H, Silvestre AM & Taveira N (2014) Microbiologia Médica Volume 1 e 2, Lidel. ISBN:9789727575763
  • Mims C, Playfair J, Roitt I, Wakelin D & Williams R (2013) Medical Microbiology, 5th ed., Mosby. ISBN: 9780808924401
  • Murray PR, Kobayashi GS, Pfaller MA & Rosenthal KS (2016) Medical Microbiology, 8th ed., Mosby. ISBN: 9780323359498
  • Tille P (2018) Diagnostic Microbiology, 14th ed., Bailey´s & Scott´s. ISBN: 9780323354820